Our objective is to detect DNA fragmentation index in Sperm in males suffering from an unknown reason infertility in case the sperm count was normal and with normal motility. A total of 100 Orient hospital patients with normal semen analysis and the selection of 30 samples in which the sperm count is normal and with normal motility. This study shows that the percentage of infertile cases who have an increased fragmentation rate of 30% is (10%) and the percentage of those who were infertile who have a fragmentation ratio have between 15-30% is (40%) of any Mediterranean person able to fertilize. The high percentage of sperm fragmentation in people who suffer from infertility refers to the need for a test to detect fragmentation with halosperm, finding of high DFI will in incurable cases point to direct referral to IVF or ICSI, instead of continuing attempts to achieve spontaneous pregnancy or using intrauterine insemination.